Thermostable exoshells
Webb29 sep. 2024 · In vitro protein folding is a complex process which often results in protein aggregation, low yields and low specific activity. Here we report the use of... Webb7 juli 2024 · We report the use of self-assembling, porous exoshells (tESs) to encapsulate and deliver an iron-containing reaction center for the treatment of breast cancer. ...
Thermostable exoshells
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WebbIn vitro protein folding is a complex process which often results in protein aggregation, low yields and low specific activity. Here we report the use of nanoscale exoshells (tES) to … Webb1 nov. 2001 · A general approach to protein folding using thermostable exoshells. Sadeghi S , Deshpande S , Vallerinteavide Mavelli G , Aksoyoglu A , Bafna J , Winterhalter M , Kini RM , Lane DP , Drum CL Nat Commun, 12 (1):5720, 29 Sep 2024 Cited by: 1 article PMID: 34588451 PMCID: PMC8481291 Free to read & use The reduced form of the antibody …
Webb13 nov. 2024 · Here we have engineered a thermostable protein nanoparticle (tES) to improve both expression and stabilization of recombinant proteins using this … Webb30 aug. 2024 · Abstract: Thermostable exoshells (tES) are engineered proteinaceous nanoparticles used for the rapid encapsulation of therapeutic proteins/enzymes, …
Webb1 okt. 1997 · Brucella usually carry two highly homologous genes (omp2a and omp2b) for porin-like proteins.In several B. abortus biovars the omp2a gene has a large deletion … WebbThermostable exoshell (tES) is a representative 12 nm NP with an 8 nm interior cavity. In this thesis, we show that the encapsulated enzymes remain highly stable in vitro. Local …
WebbArticle Title: Thermostable exoshells fold and stabilize recombinant proteins Journal: Nature Communications doi: 10.1038/s41467-017-01585-210.1038/s41467-017-01585-2
WebbA general approach to protein folding using thermostable exoshells Nature Communications 2024-09-29 Journal article DOI: 10.1038/s41467-021-25996-4 … hostein boissonsFar-UV CD spectra (260–190 nm) were recorded using a Chirascan Circular Dichroism Spectrometer (Applied Photophysics). Protein samples (0.1 mg/mL) were dissolved in 10 mM phosphate buffer and measurements were carried out at room temperature using a 0.1-cm path length-stoppered cuvette. The heat-induced … Visa mer The pRSF1b expression vector (Merck) and pBAD/HisB vector (Life Technologies) were used for cloning. Chemically competent XL1 Blue E. coli cells (catalog #RH119-80; Simply Science) and BL21 (DE3) E. colicells … Visa mer The following reagents were used: restriction enzymes NcoI, EagI, and SpeI (New England BioLabs), Expresslink T4 DNA ligase (Life … Visa mer For the expression of each clone, a single positive colony was selected from a freshly transformed plate and grown in 100-mL LB broth, with kanamycin (50 µg/mL, for a protein gene on pRSF1b vector), ampicillin (100 … Visa mer AfFtn gene was selected based on the sequence in GenBank AF_RS04235. The gene with mutations for C-terminus truncation, as well as altered charges was synthesized from … Visa mer hoste haisteWebb29 sep. 2024 · In this conversation. Verified account Protected Tweets @; Suggested users hoste haiseWebb24 aug. 2006 · Domoic acid is a potent neurotoxin that can lead to amnesic shellfish poisoning in humans through ingestion of contaminated shellfish. We have produced and purified an anti-domoic acid single-chain Fragment variable (scFv) antibody fragment from the Escherichia coli periplasm. hosteeva bienville villasWebbARTICLE Thermostable exoshells fold and stabilize recombinant proteins Siddharth Deshpande1,2,3, Nihar D. Masurkar1,2, Vallerinteavide Mavelli Girish1,2, Malan Desai1 ... hosteeva llcWebb13 nov. 2024 · Here we have engineered a thermostable protein nanoparticle (tES) to improve both expression and stabilization of recombinant proteins using this … host ekinoWebbWe report the use of self-assembling, porous exoshells (tESs) to encapsulate and deliver an iron-containing reaction center for the treatment of breast cancer. The catalytic center is paired with indole-3-acetic acid (IAA), a natural product found in edible plants, which undergoes oxidative decarboxylation, via reduction of iron(III) to iron(II), to produce free … hostein sylvie